FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_DJ27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_DJ27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26887245
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG1139616142.385008207423255No Hit
TCATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAA486692318.101233503097845No Hit
GGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATGG4892501.8196360393190152No Hit
CATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAAT2365530.879796349533022No Hit
GGGCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATG1088240.40474209983209514No Hit
TCATGCATCCAAATACAATGATCGGGCTTTATTTAAAAAAACAAAATGGG985850.36666084606288224No Hit
TCATGCATCCAAATACAATGATCGGACATGTCTCTGCGAGTATCAAATAC601190.2235967277420948No Hit
TCATGCATCCAAATACAATGATCATTCGATGGTTGGCATCCTAAAAATTT595270.22139494024025147No Hit
GCGTTAAATTCTAGGTGGAAGTCGTGCATTTATTGAAGGCCGTTTATGGT405810.15093030170997437No Hit
ATGCATCCAAATACAATGATCCAGGGAAAAAGACATTGAAGGGATGAATC385240.14327983398819774No Hit
TGGCGTTAAATTCTAGGTGGAAGAGATCATTGTATTTGGATGCATGAAGA313900.11674680689672742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTT11969100.069.642011
TCATGCA12929050.069.629061
GGCGTTA12634700.066.782042
CGACTTA12340450.066.75580664
GGTCGAC12333600.066.7400761
AGGCCGT12428750.066.7292839
GGCCGTT12430600.066.7241440
AAGGCCG12433500.066.7192738
GACTTAT12350000.066.70965
TGGTCGA12350250.066.6965660
GAAGGCC12441800.066.6944637
GGTCTCG12443750.066.68484550
GTCGACT12339200.066.6831462
ACTTATC12318400.066.6749966
GTCTCGT12443750.066.6696651
CCGTTTA12448300.066.6596342
TGAAGGC12459550.066.65562436
GCCGTTT12441000.066.6537341
TGGTCTC12448600.066.6476249
TATGGTC12463300.066.64062547
Produced by FastQC (version 0.11.5)