FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_DJ26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_DJ26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20258402
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCA513117825.328641419989594No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGATAGA483154423.849581028158095No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA248723712.277557726418896No Hit
CCATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCT13539816.683552829092837No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCAGATCGGA3090651.5256139156484307No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGAGATCGGA2877591.4204427377835627No Hit
GGGTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA1320500.6518283130130402No Hit
AATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATAAATT1103020.5444753243617142No Hit
AAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGG1047940.5172866053304698No Hit
AAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATAAAT984420.4859317136662606No Hit
CAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGG962960.4753385780378926No Hit
GTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCAT951080.46947434452134973No Hit
ATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATAAATTC949800.4688425079135067No Hit
TTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACC884010.4363670935150759No Hit
AATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGG861200.4251075677143735No Hit
CATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTC858900.4239722363096556No Hit
CATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGATAGAG842150.41570406194921No Hit
GTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAAC781470.3857510577586524No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTATCA772110.3811307525638004No Hit
CAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATAAA765030.37763590632666877No Hit
CCATTTTTATGATTGAGTGGATGCAAAATGGACCTGATTTGAACCAGATC745460.3679757169395691No Hit
ATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTCA700930.3459947136995307No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGAGATC651920.3218022823320418No Hit
TTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATA642980.3173892985241383No Hit
TCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCG639330.31558757694708595No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGAAAGA603050.29767895809353573No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGGATCGGAA568440.2805946885642806No Hit
ATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGGA560270.2765617939657827No Hit
TCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATAA495630.24465404526971082No Hit
CAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATAAATTCTT491660.24269436454069773No Hit
GGGGCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA450270.22226333547927424No Hit
TCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCATAAATTCT404210.1995270900439235No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTGTCA378810.18698908235703882No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGCCATTTTT367650.1814802569324076No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGCGATAGA340340.1679994305572572No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGAGAGA315520.15574772383330138No Hit
CCATTTTTATGATTGAGTGGAGCAAAATGGACCTGATTTGAACCAGATCG309040.15254905100609614No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTAA306340.1512162706614273No Hit
GGGTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGACGTTTCA293180.14472020053704135No Hit
TTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTCAA283100.1397444872502777No Hit
GGTTCAAATCAGGTCCATTTTGCTCCACTCAATCATAAAAATGGAGATCG264850.13073587936501604No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGATTGA229050.11306419923940694No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAC216780.10700745300641186No Hit
ATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCGAGATAGAGC203290.1003484875065664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA150.00222555269.9991150
CGTACAG150.00222555269.9991142
GTACGAT207.9322934E-569.9991140
GGTTCAA9030800.069.840611
GGGTCAA215300.069.128961
GTTCAAA9210500.068.2786942
TGCAAAG5109150.067.7261670
AATGCAA5180500.067.53992568
ACCGAGA5315850.067.427440
TGAACCG5398500.067.4265837
GCTAATC5213350.067.4164551
ATTTAGG5325050.067.40983659
GAACCGA5336150.067.4076838
AACCGAG5321700.067.40523539
CCGAGAT5215200.067.3925341
AGAGCTA5219050.067.3917748
GAGCTAA5224500.067.3911449
AGCTAAT5208100.067.38158450
ATAGAGC5186950.067.3763146
TAGAGCT5143400.067.3758847
Produced by FastQC (version 0.11.5)