FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_DJ25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_DJ25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20663478
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA877518042.46710064975509No Hit
CATTAATTTTGCTACCTGGGATCTTACAAAAATCTGAATTTTTTTTTTCA2476681.1985784774470203No Hit
TATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTT1334660.6459028823705283No Hit
GTATATTTGGCTCGAAAACTGCCCAGGTAGCAAAATTAATGAGATCGGAA1046200.5063039242474089No Hit
TTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTT900470.43577852673204387No Hit
TATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAAT804380.3892761905812758No Hit
GGATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAA689430.33364663973799574No Hit
TGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTA679550.3288652568555981No Hit
ATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTT642710.3110366996301397No Hit
GGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTTAC592550.2867619865348902No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564550.27321150873052447No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG426090.20620439598793583No Hit
ATATTTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATT419970.20324264869641018No Hit
CATTAATTTTGCTACCTGGGCAGTTTTCGAGCCAAATATACAGATCGGAA372600.18031814392523854No Hit
TTGGCTCGAAAACTGCCTTATTAACAAATTTTGGCAAAATGAAATTTTTT325890.15771304327374122No Hit
CATTAATTTTGCTACCTGGGATCTAAATATGATGTTGCCCTCCAGCTCAT315790.15282519235145217No Hit
GTATATTTGGCTCGAAACCTGCCTTATTAACAAATTTTGGCAAAATGAAA314490.15219606302482092No Hit
CATTAATTTTGCTACCTGGGATCGGAGACGGCCTGGGAATCCTGGATGTT229390.11101228941226642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATT9457200.069.7231141
CATTAAT9714600.069.64231
ATTAATT9843400.068.8013152
GGGATCT2534200.068.6704218
TATATTT9609600.068.600932
ATATTTG9721550.068.36183
TGGGATC9875150.068.33794417
ACCTGGG9931600.068.3081414
TGCTACC9939300.068.3029110
TACCTGG9936050.068.2993313
GGGATCA3576500.068.2847318
TTAATTT9918450.068.282483
GCTACCT9943100.068.2774911
CTGGGAT9895650.068.2459116
TTGCTAC9949750.068.2183469
CTACCTG9955650.068.1868512
TTTGCTA9952200.068.175558
GGATCTT913750.068.1379419
CCTGGGA9918550.068.09292615
GGGATCC1941450.067.9263518
Produced by FastQC (version 0.11.5)