FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_DJ24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_DJ24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13909901
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC299068321.500390261584176No Hit
ATGTGTTTTGACCCGAGTAATCCTAAAAAACTCTTTACATGTCCGCAAGG158445211.390821545027531No Hit
CATGTAAAGAGTTTTTTAGGATTACTCGGGTCAAAACACATTTGCAAAAA13393369.628652281565483No Hit
CATGTAAAGAGTTTTTTAGGATCAATATCACTTTAAAATAAGGTACAGTC12208318.776705168498324No Hit
CATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATTTGC2848102.047534342624006No Hit
ATGTGTTTTGACCCGAGTAATCCGATCCTAAAAAACTCTTTACATGAGAT1393711.0019553697758166No Hit
GTGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC1370520.9852837917394237No Hit
ATGTGTTTTGACCCGAGTAATCCTAAAAAACTCTTTACATGAGATCGGAA1158020.832514911500808No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGCCCACCTGTCAATCC1099440.7904010244213815No Hit
CATGTAAAGAGTTTTTTAGGATCGGATTACTCGGGTCAAAACACATAGAT934580.6718811298513195No Hit
GTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAG815610.5863521242890226No Hit
TGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTA794960.5715065836917171No Hit
ATGTGTTTTGACCCGAGTAATCCGATCCTAAAAAACTCTTTACATGTCCG626020.45005352662107373No Hit
CATGTAAAGAGTTTTTTAGGATTACTCGGGTCAAAACACATAGATCGGAA599600.43105986160505383No Hit
TTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAGG535590.3850422803152948No Hit
GTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGT420700.30244643725357934No Hit
ATGTGTTTTGCCCCGAGTAATCCACAACAACAACGACCACCTGTCAATCC403120.28980795765548584No Hit
TGTGTTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCG390100.2804477184992187No Hit
TTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAGGA354950.25517794842680763No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACAACGACCCCCTGTCAATCC286690.20610498953227632No Hit
CATGTAAAGAGTTTTTTAGGATTAGGATTACTCGGGTCAAAACACATTTG272240.19571670567605048No Hit
ATGTGTTTTGACCCGAGTAATCCACAACAACACCGACCACCTGTCAATCC267540.19233781750136106No Hit
TTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAGGAA255060.1833657910289944No Hit
ATGTGTTTGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCG240970.17323631562870218No Hit
ATGTGTTTTGACCCGAGTAATCCTAATCCTAAAAAACTCTTTACATGTCC236970.17036066611832823No Hit
GACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAGGAATT233870.16813203774778843No Hit
TGACCCGAGTAATCCACAACAACAACGACCACCTGTCAATCCGTAGGAAT224720.16155398949280805No Hit
GTGTGTTTTGACCCGAGTAATCCACAACAACAACGCCCACCTGTCAATCC186050.13375364785126798No Hit
CATGTAAAGAGTTTTTTAGGATTACTCGGGTCAAAACACATTTGAAAAAA178190.12810299656338314No Hit
TGTGTTTTGACCCGAGTAATCCTAAAAAACTCTTTACATGTCCGCAAGGC177560.12765008176549927No Hit
GTGTTTTGACCCGAGTAATCCTAAAAAACTCTTTACATGTCCGCAAGGCT177350.12749911016620463No Hit
ATGTGTTTTGACCGAGTAATCCACAACAACAACGACCACCTGTCAATCCG170480.1225601821321374No Hit
CATGTAAAGAGTTTTTTAGGATCACTGGTTTGTCGCTAGAAACTTTCTGT166270.11953356102246881No Hit
ATGTGTTTTGACCCGAGTAATCCTAAAAAACTCTTTACATGTCAGCAAGG158810.11417047468562141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTAA6611300.069.833761
ATGTGTT5633450.069.7378541
ATGTAAA6678300.069.176872
TGTGTTT5979600.068.8608552
TGTAAAG6760200.068.4690253
GATCAAT1459450.068.439220
TAAGGTA1353200.068.3567139
GGATCAA1808150.068.3226319
GTAAAGA6824900.068.2912454
AATAAGG1370500.068.2599737
ATAAGGT1360100.068.2518238
CACTTTA1363350.068.1894829
TAAAGAG6842450.068.172875
ATATCAC1366650.068.0495925
AGGATCA2632450.068.041218
AAGGTAC1357600.068.0165740
TATCACT1366150.068.01276426
AAAGAGT6855350.068.0017246
TTTAGGA6867600.067.94009415
TTTTAGG6885500.067.9083514
Produced by FastQC (version 0.11.5)