FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_DJ23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_DJ23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22034344
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTC813509336.920059884696364No Hit
AATAAAATGTTAACGGTAAGCTTAGTTTGGCACCACTTGTTTTTATAACA21115729.583094463806137No Hit
AATAAAATGTTAACGGTAAGCTTGCGAAGACAACGTTTTTTCACCATTCT19819938.995017051562778No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG3242271.4714620049500906No Hit
AAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGC2862821.2992535652524986No Hit
AGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGCA2479311.1252025474413943No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1225790.5563088240793554No Hit
AATAAAATGTTAACGGTAAGCTTCGAAGATCTTAGAAGTAGCTCCTGTAG1178080.5346562620607176No Hit
GAAAGTGGTAAGGTTGGAGACCGCGGGCTAAATGATGGCCTGAACCATGT1000170.4539141260570317No Hit
GTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGCAT622970.28272681955042545No Hit
AATAAAATGTTAACGGTAAGCTTCGAGCAGTTACAGAAAACCTACAGTAC552140.25058154669819077No Hit
AATAAAATGTTAACGGTAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG527710.23949430942895328No Hit
ATAAAATGTTAACGGTAAGCTTAGTTTGGCACCACTTGTTTTTATAACAT422400.19170073772107762No Hit
ATAAAATGTTAACGGTAAGCTTGCGAAGACAACGTTTTTTCACCATTCTG421590.1913331297723227No Hit
AATAAAATGTTAACGGTAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA372550.1690769645785688No Hit
GAAAGTGGTAAGGTTGGAGAAGAAGCTTACCGTTAACATTTTATTAGATC283660.128735395980021No Hit
AAATGTTAACGGTAAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAG269650.12237713997748241No Hit
AAATGTTAACGGTAAGCTTAGTTTGGCACCACTTGTTTTTATAACATTTT262030.11891890223734367No Hit
GGAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTC260230.11810199568455498No Hit
AATAAAATGTTAACGGTAAGCTTACAACATCCAGGATTCCCAGGCCGTCT233570.10600270196380705No Hit
AATAAAATGTTAACGGTAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT223440.10140533341950185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAGTG8789400.069.6623841
TAAAATG11302650.067.708743
AAAGTGG9155150.066.99532
AAAATGT11454400.066.8276144
AAATGTT11561800.066.177335
AATGTTA11568950.066.105266
GTTAACG11573950.065.975129
TGTTAAC11578550.065.934858
TAACGGT11583400.065.9345911
ATGTTAA11585250.065.926337
CGGTAAG11590700.065.9211214
AACGGTA11586300.065.920512
ACGGTAA11582350.065.9183513
TTAACGG11582750.065.9156310
GGTAAGC11588050.065.8985815
GTAAGCT11589350.065.87820416
ATGCATA2345450.065.79687570
GAACCCG2275550.065.7757655
TAAGCTT11610900.065.74538417
GCACCAC2326650.065.6923730
Produced by FastQC (version 0.11.5)