FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_AKM39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11001637
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6428875.8435576451031785TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC659010.5990108562934771TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC226810.20616022870051068TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGC113300.10298467400805897TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC841900.067.4300544
GTATGCC850850.067.29253445
TGCCGTC821700.067.2817148
TATGCCG839300.067.2803146
ATGCCGT819800.067.262647
GCCGTCT803450.067.23740449
CTCGTAT754150.066.9686842
TCGTATG821950.066.818443
AGTCACC947600.066.76739528
CCTTGTA939750.066.71805633
GTCACCT944300.066.6449129
CACCTTG946250.066.3633331
ACCTTGT944500.066.3417632
GTAATCT789550.066.2445837
TGTAATC835950.066.13479636
CAGTCAC963700.066.0441927
CCGTCTT833550.066.0102950
CCAGTCA961050.065.9352626
CTGCTTG739900.065.893657
TCTCGTA771300.065.73373441
Produced by FastQC (version 0.11.5)