FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_AKM38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10918724
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2894392.6508500443824756TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC348550.066.3741844
ACGGCTA353450.066.1471732
CGGCTAC352000.065.8926733
ATGCCGT348600.065.872747
TATGCCG350250.065.8521746
GTATGCC353350.065.5616845
CACGGCT355950.065.4662731
TGCCGTC349650.065.3445648
GCCGTCT347950.065.1608849
CTCGTAT347750.065.158142
GGCTACA352200.065.1099534
TCACGGC359550.064.956830
GTCACGG361050.064.9486829
TCGTATG353850.064.74743
AGTCACG367350.064.006928
GCTACAT355300.063.7735135
CCGTCTT361300.062.9856850
CTGCTTG354450.062.15950457
CATCTCG357050.062.1376139
TCTCGTA362000.062.08074641
Produced by FastQC (version 0.11.5)