FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_AKM37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12998443
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1054920.8115741246855488TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC316060.24315219907491994TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGC164050.12620742345833266TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC354950.064.7338631
ACTAGCT357600.063.95073732
TAGCTTA358850.063.34759534
CTAGCTT360800.062.9858233
TCACTAG371250.062.23106430
GCTTATC357850.061.6076336
TATGCCG184000.060.92605246
CGTATGC171300.060.66200644
GTATGCC192850.060.65278245
CAGTCAC383300.060.32102627
CCAGTCA388250.059.55218526
TATCGCG91900.059.41184239
ATGCCGT161850.059.3599647
AGCTTAT380550.059.2111135
GCCGTCT163550.058.74294749
TGCCGTC176850.058.6989148
CTTATCT250700.058.3281337
CTCGTAT163450.058.26497742
TCCAGTC396850.058.2442425
ACTCCAG398100.057.96531723
Produced by FastQC (version 0.11.5)