FastQCFastQC Report
Wed 27 Mar 2019
H5NLTBGXB_n01_AKM36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13378193
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC183504113.716658146582278TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC278060.20784570831053192TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC183430.13711119281953849TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC144010.10764532997842086TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2112750.068.44842544
ATGCCGT2107950.068.3950947
GTATGCC2116150.068.3781545
TATGCCG2113550.068.33806646
CTCGTAT2059050.068.2601142
GCCGTCT2102850.068.2580549
TGCCGTC2107800.068.2554948
TCGTATG2113700.068.2057143
AGTCACG2127650.068.0663928
GATCTCG2020600.068.0122339
CACGATC2115800.067.9546631
CCGTCTT2117150.067.85155550
GTCACGA2126450.067.84968629
CGATCAG2119000.067.82733
CTGCTTG2103650.067.7911957
CAGATCT2022250.067.7871337
AGATCTC2025450.067.76642638
TCTCGTA2057600.067.64822441
ACGATCA2130600.067.57271632
TCACGAT2135300.067.5127430
Produced by FastQC (version 0.11.5)