FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10861005
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC425840.39208157992745607TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC169260.15584193175493427TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC254600.059.5241529
CCAGTCA262050.057.95236626
CAGTCAC265000.057.426127
AGTCACA266950.057.08528528
TCCAGTC268550.056.68000825
ACACTTG269250.056.0641732
ACTCCAG273100.055.81361423
TATGCCG92300.055.58965346
GTATGCC97350.055.25856845
CGTATGC84700.055.24712444
TCACACT277700.054.5724830
CACTTGA277200.054.41839233
GAACTCC281600.054.2912221
CTCCAGT283000.053.93483424
ACGTCTG287800.053.65749415
ATGCCGT75850.053.43362447
CGTCTGA288750.053.40896616
CACACTT284650.053.15397331
CACGTCT293500.052.6154214
TGCCGTC86600.052.45881748
Produced by FastQC (version 0.11.5)