FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12029672
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC868510.7219731344296004TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC183010.15213216120938294TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC125750.10453319092989402TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC138050.060.21270844
AGTCACC244500.059.34873228
TATGCCG143750.059.0912146
GTATGCC149250.058.71931545
TGCCGTC142750.058.03408448
ATGCCGT132800.057.55930747
GCCGTCT134750.057.50555449
CAGTCAC257000.056.81619627
CTCGTAT132500.056.6858742
ACCAGAT254750.056.5761232
GTCACCA257900.056.2650829
TCGTATG130550.055.78997443
CCAGTCA261300.055.57430326
CCAGATC256950.055.43789733
TCCAGTC268950.054.26706725
TCACCAG267700.054.04842830
CACCAGA268200.053.93461631
ACTCCAG275650.052.8854323
CAGATCA269500.052.6095334
GAACTCC280650.052.1182721
Produced by FastQC (version 0.11.5)