FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10378315
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC6119445.896371424455705TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC706350.067.55147644
ATGCCGT703400.067.4068647
TATGCCG707450.067.38212646
GTCACGC721200.067.34466629
GTATGCC711700.067.2944845
GCCGTCT696750.067.15607549
TGCCGTC704300.067.15176448
CACGCCA718000.067.00136631
CTCGTAT703750.066.98044642
TCACGCC719300.066.9629930
TCGTATG709500.066.8421343
AGTCACG732600.066.8270228
CCGTCTT711300.066.04314450
TATCTCG717300.066.03232639
GCCAATA728950.065.8700634
ACGCCAA730750.065.8323432
CGCCAAT728850.065.8118733
CAGTCAC745200.065.706827
CCAGTCA744500.065.6983826
TCTCGTA722050.065.5058341
Produced by FastQC (version 0.11.5)