FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11940644
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC5377614.503618062811353TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC3687233.087965774710309TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC984670.8246372641207627TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC973920.815634399618647TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATTC224070.1876531952547953TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTTTGC207790.17401908975763786TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTTTGC199590.16715178846300083TruSeq Adapter, Index 5 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGG185720.15553599956585257TruSeq Adapter, Index 5 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGG169030.14155852900396326TruSeq Adapter, Index 5 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATTC127500.10677816037392958TruSeq Adapter, Index 5 (97% over 48bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1723050.068.0820313
ACGTCTG1720500.068.05038515
GCACACG1730150.068.0289211
CACGTCT1722800.068.0082914
CACACGT1731600.067.9601212
CGTCTGA1725750.067.8679816
GTATGCC1284600.067.71335645
TATGCCG1254650.067.6922646
GCCGTCT1138100.067.67435549
ATGCCGT1180350.067.6567547
TGCCGTC1192800.067.6313248
CGTATGC1261950.067.62794544
ACTCCAG1701500.067.45907623
AGCACAC1746700.067.4404610
GAACTCC1709350.067.43622621
GATCGGA1726100.067.413491
GTCACAC1703300.067.3649929
CCAGTCA1702950.067.3591226
CAGTCAC1712250.067.3381627
CACAGTG1702400.067.32452433
Produced by FastQC (version 0.11.5)