FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11992663
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC160855613.412834163688247TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC414700.3457947580116276TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGC124330.10367171995077323TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGC121950.10168717323250057TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1986950.068.35751
GCACACG2003850.068.0763611
CACACGT2007450.067.99090612
ACACGTC2003950.067.9611913
ACGTCTG2006600.067.8693215
TCGGAAG2031400.067.84373
CACGTCT2006450.067.7851514
CGTCTGA2009450.067.73991416
CGGAAGA2043000.067.5784154
AGCACAC2021300.067.5163610
AGAGCAC2039600.067.244938
CGTATGC1976900.067.21068644
TATGCCG1972850.067.1198146
ATGCCGT1963050.067.1054347
CTCGTAT1898950.067.0925442
ACTGACC1965050.067.0852332
GTATGCC1983250.067.0766745
ATCGGAA2054050.067.063212
GCCGTCT1947650.067.0160749
GAACTCC2005450.067.0073821
Produced by FastQC (version 0.11.5)