FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11316490
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC6346265.607975617881516TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC718250.6346932661982646TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC291640.2577124178963619TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGG189420.1673840563637665TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC182810.16154302261566972TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATGC143570.12686795994164268TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG1059200.067.8941231
ACTTAGG1058850.067.8306132
CGTATGC811150.067.4014744
GTATGCC831650.067.2298245
TATGCCG813450.067.223846
ATGCCGT780550.067.169647
CTCGTAT750100.067.1060742
AGGCATC983500.067.10577436
ACGTCTG1084800.067.011715
TGCCGTC794950.066.9919148
CGTCTGA1086400.066.89719416
GGCATCT842950.066.7811637
TCGTATG751650.066.7441943
CACGTCT1090350.066.70270514
ACACGTC1091600.066.7003313
GCACACG1092950.066.6531611
GCCGTCT766650.066.6480849
CAGTCAC1079700.066.6153427
TTAGGCA1065650.066.5832734
CCAGTCA1077600.066.5440826
Produced by FastQC (version 0.11.5)