FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10623618
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC9585619.022924205294279TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1249171.175842354271398TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC585850.5514599640160254TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG395880.3726414108639825TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC326250.30709876804681796TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC236740.2228431029805477TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC216000.20332056367237603TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTGC153660.14463998987915416TruSeq Adapter, Index 2 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC130950.12326309172637796TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGG114730.10799522347283196TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGG113690.1070162726107057TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATGC109840.10339227182302677TruSeq Adapter, Index 2 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1183500.068.1833844
GTATGCC1229650.068.13483445
ACGTCTG1676100.068.0986115
ACACGTC1677750.068.0931113
TATGCCG1196750.068.07773646
CACACGT1680800.068.06949612
GCACACG1681900.068.06035611
CACGTCT1676850.068.04310614
GTCACCG1650750.067.9619529
ATGCCGT1121950.067.9558747
CTCGTAT1127300.067.9376242
AGTCACC1656100.067.9114628
CGTCTGA1680500.067.9033216
TCGTATG1090400.067.8999643
CGATGTA1639150.067.85966534
TGCCGTC1156200.067.8196348
CCGATGT1640400.067.8015533
GTATCTC1277200.067.7438638
CCAGTCA1664950.067.6919726
ACTCCAG1665150.067.6841623
Produced by FastQC (version 0.11.5)