FastQCFastQC Report
Tue 26 Mar 2019
H5NLTBGXB_n01_AKM28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLTBGXB_n01_AKM28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11553453
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC156730613.565693303984533TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATGC218660.18925943611836218TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC208790.1807165355673321TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC206950.17912393809885235TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC187350.16215931289113306TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1893650.068.1101911
ACACGTC1893400.068.09263613
CACACGT1894900.068.0695512
ACGTCTG1890300.068.0483915
CGTATGC1860500.067.950544
TATGCCG1858650.067.899546
CGTCTGA1895200.067.88811516
CTCGTAT1785800.067.87263542
CACGTCT1896050.067.8697314
ATGCCGT1856400.067.8592547
GTATGCC1864700.067.84437645
TGCCGTC1852000.067.7899148
TCGTATG1854300.067.7511243
GATCTCG1737800.067.73334539
CGATCTC1732350.067.7201538
CACGATC1726750.067.7127636
GCCGTCT1848050.067.6772349
ACGATCT1725850.067.6750737
AGCACAC1914900.067.51337410
GAACTCC1881150.067.4927521
Produced by FastQC (version 0.11.5)