FastQCFastQC Report
Tue 26 Apr 2022
H5NLHDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NLHDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957813670
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA855299234.368644693343059No Hit
TCCGGAAG850986854.346618184558902No Hit
TCGGCGTC696937753.5597756859057994No Hit
GTAATCTT689166853.5200839618205344No Hit
ACCCTCCT688627753.5173303800662503No Hit
TGCTCGTA633351523.2349938592470857No Hit
CTCCTCTA628330423.2093473941266333No Hit
AGTTCGGC626937933.2022349195263304No Hit
AACCGTAA608872973.1099638302147516No Hit
CCAGGAGC607071703.1007634143243057No Hit
ACTGCTCG606799003.099370534071304No Hit
ACGACATT605157073.0909839852124437No Hit
GCTGAATT586750402.996967530623075No Hit
CACGTCGG574187142.9327976854916944No Hit
ATGCTCCG571239552.9177421669550405No Hit
GAAAGGGT570833412.915667710094189No Hit
CACTCGGA562096502.8710418596678817No Hit
GGTAACGC549754812.8080037361267376No Hit
AACTGGCG545646082.7870174182612586No Hit
TGGTAAAC540175662.759075944137217No Hit
TGAAGTAC539797042.757142052236258No Hit
TGGAATTA532832142.7215671652757436No Hit
CTAAACGG528880622.7013838349591257No Hit
GTTGCAGC527220952.6929066748216135No Hit
TGTCAGAC526569842.68958097529271No Hit
CAATGGAG513484142.6227426433282592No Hit
GATCTCAG511477862.6124950899949533No Hit
TTGGCATA488361512.49442282216775No Hit
CCACTTAT472529992.413559560037192No Hit
ATGAATCT450820052.3026708665283757No Hit
GTATGTCA433545322.2144360653074813No Hit
GGTTTACT424187832.166640454604651No Hit
GGGGGGGG261984341.3381474652794716No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)