FastQCFastQC Report
Thu 27 May 2021
H5NKYDRXY_n01_dei075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NKYDRXY_n01_dei075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3898927
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGGT1006712.5820180783071853TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTT622391.5963109850479373TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGGT483161.239212737247966TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGTT365150.9365397197741839TruSeq Adapter, Index 21 (97% over 40bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275830.7074510499940112No Hit
TAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214840.5510233969499814No Hit
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150040.38482382460610315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGGG114040.29249072885950417TruSeq Adapter, Index 21 (97% over 40bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70980.182050087113711No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTG62240.15963366331300893TruSeq Adapter, Index 21 (97% over 40bp)
TGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61010.1564789492083335No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGGG55460.14224426361406614TruSeq Adapter, Index 21 (97% over 40bp)
CTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50730.13011272075624908No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTGT46310.11877626844513887TruSeq Adapter, Index 21 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)