FastQCFastQC Report
Thu 27 May 2021
H5NKYDRXY_n01_dei074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NKYDRXY_n01_dei074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633341
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1347035.115288904855087TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT731382.7773843190076786TruSeq Adapter, Index 20 (97% over 45bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344801.3093632765372962No Hit
TAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239580.9097948195847024No Hit
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160280.6086564558103186No Hit
TGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85330.3240370312845925No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59950.22765756504759543No Hit
CTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44580.16929064636900423No Hit
TGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36590.13894896255365333No Hit
TCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28870.10963259220890875No Hit
TGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26400.10025287268150991No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)