FastQCFastQC Report
Thu 27 May 2021
H5NKYDRXY_n01_dei061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NKYDRXY_n01_dei061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24948209
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA713790.28610871425680295No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC568880.22802438443577253TruSeq Adapter, Index 12 (100% over 50bp)
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC457710.18346407150910113No Hit
CGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTC388930.15589495823127023No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG260830.10454858703484486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)