FastQCFastQC Report
Fri 2 Jun 2023
H5NKTDRX3_n01_3125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NKTDRX3_n01_3125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20174692
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC382730.18970797670665804No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG298760.14808652345225395No Hit
GTATGTGCGCAGCTCGCGTTGCATTTTCGGAAGCGCTCGTTTTCGGAAAC279550.13856469283397238No Hit
ATATAGAACATAGCAAAGAGCTGCTTTTGAGCAATGTTTGTGGAAGCGGT223710.1108864512033195No Hit
TTACAGTCCGGTGCGTTTTTGGTTTTTTGAAAGTGCGTCTTCAGAGCGCT208490.10334234594510787No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)