FastQCFastQC Report
Thu 27 Jul 2023
H5NJNDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NJNDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071339101
Sequences flagged as poor quality0
Sequence length24
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGATGCTCGTGTAGATCTCGGT27726849825.880554321334344No Hit
ATCAGTCTAAGTGTAGATCTCGGT344774943.2181681754934846No Hit
CAGGGTTGGCGTGTAGATCTCGGT296768122.7700671031514976No Hit
AGGTAACACTGTGTAGATCTCGGT244152352.2789455716878573No Hit
TTAGATGCTCGTGTAGATCTCGGT234053492.1846816734452412No Hit
AGATCTCGGTGGTCGCCGTATCAT190701941.7800334163291218Illumina Single End PCR Primer 1 (100% over 24bp)
TTCAGTCTAAGTGTAGATCTCGGT31529310.29429813558163037No Hit
GGGGGGGGGGGGGGGGGGGGGGGG21126750.19719946728612867No Hit
TGGTAACACTGTGTAGATCTCGGT20420880.1906107970943926No Hit
TGATCTCGGTGGTCGCCGTATCAT14991550.13993281852596173Illumina Single End PCR Primer 1 (95% over 24bp)
AGGTATCACTGTGTAGATCTCGGT13380180.1248921092071669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)