FastQCFastQC Report
Thu 27 Jul 2023
H5NJNDRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NJNDRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071339101
Sequences flagged as poor quality0
Sequence length10
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCCCTGT29924974927.93230908128686No Hit
TCACACTCAT13929803113.002235321195469No Hit
ATTGGGAAAT11488681410.723664794159324No Hit
CAGTCTGGAT1003397589.365826180183449No Hit
GGCATACTAT439640384.103652891877415No Hit
CACTACGAAA379269463.540143915647115No Hit
GTGGATCAAA310734272.9004287224274474No Hit
GGGGGGGGGG299232622.793071024110787No Hit
GTAACATGCG295533412.7585421807544015No Hit
TGTAGCGGAT212549081.9839570851246286No Hit
CTGCAGCCAT205916551.922048302052965No Hit
CCTACCATAT192573481.7975025817712593No Hit
AGCCCTTTAT190392821.7771480553849404No Hit
CAAGTCCAAT187262891.747932935754951No Hit
AAAGCATAAT178063951.6620689922900518No Hit
AAACGGCGAT174377371.627658038778144No Hit
TCTTAGGCAT172252181.6078212756280235No Hit
TTGTAAGAAT169845761.5853594799393027No Hit
GGCGTTTCAT168877581.5763223786228635No Hit
GTGAGAAGAT143675291.3410813613158696No Hit
GCCTTTATAT92926480.8673862450578101No Hit
ATTGGAAATC15497810.14465830646463076No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)