FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18413940
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357860.19434189532495488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT37850.09.5217041
GTCGAAT25500.09.4678691
GGCGAAT29650.09.0867661
GGGAGAT89000.09.081661
GCGAAAT36800.09.0327321
GGGAAAT57000.08.9623381
GCGGAAT39200.08.7474871
GGAAAAT78300.08.579911
GGCAAAT64100.08.5700821
ACCTAGG32300.08.44708410
TACCTAG32400.08.3130519
ATACCTA32450.08.3002428
GGCAGAT86000.08.0558261
GTCCGAT27350.07.8039741
GTGAAAT65600.07.68072031
GTCGGAT60500.07.5763361
GGAGAAT69350.07.5681221
GCGGGAT61550.07.56078531
CGGAAAT31050.07.5501661
GGCCAAT51850.07.4906161
Produced by FastQC (version 0.11.2)