FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19761962
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365880.18514356013841135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT94800.09.3757841
GCGGAAT39500.09.2133691
GTCCGAT29650.09.20561
GGCAAAT73600.09.1286311
GTCGAAT28900.08.9601671
ATACCTA34050.08.8353548
GGGAAAT66650.08.50544551
ACCTAGG34300.08.36302610
TACCTAG32650.08.3570719
GGGGAAT41900.08.3515711
GCCAGAT92200.08.3118061
GGGTAAT27400.08.3012791
GGGCAAT48100.08.0753271
GGCGAAT30350.08.0708921
CGCTTAT29300.07.99942163
GCGAAAT40600.07.67089751
GGCAGAT95850.07.59371
GTGGAAT69200.07.43350171
GGCCAAT57600.07.41172931
TCGCTTA23400.07.3256832
Produced by FastQC (version 0.11.2)