FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15982563
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542500.3394324176917056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCTA27950.09.8871578
TACCTAG27250.09.8844029
GGGAAAT52800.09.8082731
GGCAAAT57150.09.7964451
ACCTAGG26400.09.67264210
GTCGAAT22450.09.5077611
GCGGAAT29900.09.4793511
GGGGAAT33200.09.0641091
GCCGAAT29250.08.6133371
GGAAAAT76100.08.0467061
GTCAAAT50900.08.0432741
CGATCTA28750.08.0302094
GGCGAAT25100.07.9463171
GGGAGAT75800.07.8939011
GTTGGCT61750.07.82000261
GGGAATT38750.07.76589541
TAGGCAC33000.07.738283613
AATCCAC59100.07.6352685
TCGCTTA20700.07.60472062
CGCTTAT23550.07.5753033
Produced by FastQC (version 0.11.2)