FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17561196
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1364130.7767865013294083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278150.15838898444046748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT56400.09.8037261
GGAAAAT83550.09.8012761
GGCAAAT66350.09.2829271
CGCTTAT24100.08.9994753
GGGGAAT37250.08.9250471
GTGAAAT65050.08.7690691
GTCGAAT26450.08.7323561
GGGAGAT83300.08.5283351
GGCGAAT26050.08.4634231
CGCGAAT18350.08.3913111
AATCCAC61100.08.244495
GTTGGCT66650.08.1385071
ACCTAGG28400.08.12953410
GTCGCAT32400.07.99281741
TACCTAG28350.07.77367359
GTCGATT36650.07.6388711
GGCAGAT78800.07.59421831
CTGGAAT76800.07.5185821
GGCAATT47500.07.44116541
GGGAAAC52350.07.3534171
Produced by FastQC (version 0.11.2)