FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19223041
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294900.15340965042939878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAT26750.09.284773
ACTTAGT22300.08.62766556
TCGCTTA21700.08.2218082
GGCAAAT70400.08.1517791
ACCTAGG31400.08.13273513
ATACCTA31150.08.08566211
GGGGAAT44400.08.0389341
GGCGAAT32950.07.9650241
CGGAAAT32750.07.9068161
GGGAGAT92100.07.90287161
TACCTAG34100.07.89911812
GCGAAAT39250.07.7564111
GGGAAAT65200.07.6748461
GTGAAAT77550.07.5355661
GTCGAAT28450.07.50289341
GGGAAAC61250.07.4842181
CGCGAAT19500.07.3575041
GGCAGAT90550.07.3425411
GGCCAAT59100.07.3420181
GTCCGAT28450.07.2568971
Produced by FastQC (version 0.11.2)