FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18802863
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500140.2659914077978444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG32500.09.68715313
GTCAGAT56350.09.0657661
ATACCTA36650.08.87651611
GGCAAAT78050.08.8315911
GTCGATT43950.08.5982581
TACCTAG36000.08.55100512
GGAAAAT98650.08.4770821
GGGAAAT70950.08.3345061
CCTAGGC40650.08.08919814
GGGCGTA41800.07.95598637
GGAAGAT144300.07.68667551
GTCGAAT28050.07.6092631
GGCCAAT60750.07.6027941
GTCAAAT68700.07.58883571
CGATCTA32800.07.572094
GTCGGAT49000.07.5692941
GGGAGAT92600.07.5572651
CCCGGAT38500.07.5433281
TAGGCAC41350.07.529307416
CCGGAAT37200.07.52476021
Produced by FastQC (version 0.11.2)