FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16570016
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481540.290609254692331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG27750.08.82420513
GGAAAAT85850.08.8038381
GGGAAAT56550.08.7864681
GGGGAAT37700.08.5389611
TCGCTTA20550.08.5115622
GGCAAAT64250.08.3869911
CGGAAAT31350.08.3710961
ATACCTA30250.07.97929111
GTCGATT34050.07.91283561
GTGAAAT69100.07.89961
TACCTAG30650.07.875180212
GTCAGAT48650.07.839751
GTCGGTT28250.07.80334231
GGAAAAC75850.07.796321
GTCGAAT27400.07.79000661
CGCTTAT26050.07.7886033
GGAAGAT119300.07.77255771
GTCGCAT31400.07.68914371
CGATCTA28700.07.67885734
CCGGAAT31900.07.56862451
Produced by FastQC (version 0.11.2)