FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16992076
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1638680.9643789258004732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254520.1497874656398665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGCT62850.09.9661311
GGCAAAT64150.09.27323151
GGGGAAT34050.09.0436641
GCCGAAT26350.08.8976031
ACCTAGG28550.08.69934713
CGGTTCG51850.08.56877521
ATACCTA30050.08.26512711
GGGAAAT54750.08.1809681
GTCGAAT26250.08.1316631
GGAAAAT81450.08.0769171
TACCTAG29800.07.982281712
GTGAAAT66800.07.96243571
GGAAGAT119700.07.8638821
GGGAGAT79550.07.8299441
TCGCTTA21100.07.79231362
ATTTTTT169300.07.77158741
GTCGCAT30500.07.68694541
CGCTTAT23750.07.6590593
TTGGCTT100300.07.6033782
GGCCAAT50750.07.58464861
Produced by FastQC (version 0.11.2)