FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14991003
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT893890.5962843180006034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207180.13820289409587871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG27000.010.1056410
ATACCTA26800.09.658958
CGCGAAT16500.09.3314271
TACCTAG27900.09.27813159
CGCTTAT20950.09.0167783
GTCCGAT20450.08.8979381
GGAAAAT68900.08.8878781
GTTTAAT25300.08.4370111
TATCGGT28700.08.41499236
GGGAAAT46400.08.3711351
GTGAAAT56800.08.1937481
GTTGGCT54750.07.9892351
GGGTAAC27350.07.93256571
AATCCAC54300.07.85959635
GGCAAAT50750.07.7915111
CGCAAAT24100.07.69552331
GTCAAAT45650.07.66546541
GGGAGAT66300.07.6002571
GCCAGAT63750.07.57492261
CTATCGG30600.07.5491935
Produced by FastQC (version 0.11.2)