FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15668322
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA25550.010.1316932
GTCGAAT28150.09.9443771
GGGGAAT36250.09.7494331
CGCTTAT27400.09.5751673
GGCGAAT27500.09.4159681
GTCCGAT23200.09.351251
CGGGAAT24450.09.3025171
GTCGGAT41400.08.7902041
GCCAGAT83100.08.126851
GGGAAAT57350.07.992891
GGCAAAT57250.07.8846091
CGCGAAT18500.07.75493341
TCGAATT31150.07.636462
CGGCAAT45500.07.536691
GGAAAAT80000.07.3920121
GTGAAAT58950.07.36044071
CTGGAAT81100.07.3348971
GTAAATT27700.07.32679751
CTCGTAT28200.07.3209741
GGAGAAT70900.07.30434751
Produced by FastQC (version 0.11.2)