FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18378166
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAA28700.017.32298749
CTATTAG30750.015.93810246
ATTAGTA33850.014.79070948
TATTAGT34350.014.57446247
GTTAGTT32350.013.8462491
TAGTCAT53900.013.8241476
TCGCTTA36650.013.3634722
ATGCGTG50600.013.2738916
CGCTTAT39950.013.1349343
GTCATAT57750.012.9630088
CTGACAT70750.012.8106091
CTATTAA34500.012.67486112
TAATTCG55600.012.53299756
CATGCGT55100.012.38038355
GTAACAC41800.011.81092752
TCATATG64900.011.8043869
TTAAGCC65050.011.62026129
GTCCCTA37950.011.5223818
GTCCTAT44950.011.44771743
GCCAGTA74250.011.4055261
Produced by FastQC (version 0.11.2)