FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22232543
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG32350.09.63403456
CTATTAG28800.09.35990546
GTCGGAT63700.08.95391
GGCAAAT90350.08.9463911
TCGCTTA34600.08.8971412
TTAGTAA32800.08.85976949
GTCGAAT39150.08.7590781
GGCGAAT38750.08.5785911
CGCTTAT37500.08.4886843
ATTAGTA34100.08.3166248
CGGGAAT31800.08.1427041
GGGAAAT83750.07.9801791
GTCGGGA52000.07.9404071
GGTCCTA36350.07.801133642
TATTAGT37400.07.769645747
GGGGAAT52250.07.7684751
CTGACAT99150.07.62298541
GGAAGAT169100.07.53221
GGAAAAT110400.07.4800931
GTCCTAT38850.07.47923843
Produced by FastQC (version 0.11.2)