FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20911967
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAA23750.014.30016149
CTATTAG25050.013.41633646
TCGCTTA48450.012.0577692
ATTAGTA28850.011.40789448
TATTAGT29850.011.37692447
CGCTTAT52200.011.1241083
GTCCTAT32750.010.68913443
CCTATTA34100.09.95769245
GTCGAAT34750.09.7680671
GGCGAAT32450.09.7055361
GGTCCTA35400.09.69129642
TAATTCG33200.09.07085556
CTATGCG32850.08.84738754
TCCGTTA35050.08.7827319
GGCAAAT67000.08.6701181
CGGGAAT31350.08.4833581
GGGAGAT98400.08.3217081
GCGATTT49300.08.3048291
CTGACAT69650.08.2397581
GAAAAAT57350.08.1764181
Produced by FastQC (version 0.11.2)