FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19190255
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA35500.010.6425682
CGGGAAT26350.010.0923841
CGCTTAT38450.09.6438473
GAAAAAT56900.09.2859321
GGCGAAT31200.08.5235361
GGGGAAT42900.08.4827541
GTCAAAT47750.08.0608371
GCGGAAT37000.08.0385551
GGCGTAT22150.07.89872651
TTAGTAA16950.07.84925249
GTCGAAT29450.07.723051
CTATTAG18200.07.69381746
GTTGGCT71450.07.6887931
GGGAGAT92300.07.6200031
GCCAAAT71100.07.52978661
GGGAAAT58150.07.46161371
GCCAATT50050.07.41075661
GGGAATA29000.07.3583912
GGAAAAT68100.07.2962891
GCGAAAT29950.07.243621
Produced by FastQC (version 0.11.2)