FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17495500
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT25850.09.6113681
CTATTAG17400.09.4558146
GAAAAAT51550.09.2999221
GCGGGAT65800.09.0940561
GCGGAAT40850.08.9946581
GCGATTT36900.08.6298331
TAATTCG26550.08.44125756
CTATGCG23450.08.3619454
ATTAGTA21050.08.31620448
CGCTTAT35600.08.2539673
GCGGTAT23600.08.1552661
TATTAGT21650.08.08517747
GCGAAAT27350.08.060661
GGCAAAT51400.08.0335271
TCGCTTA35350.07.9166512
GGCAATT44150.07.8467871
GTCGAAA44950.07.78498361
AATTCAC36500.07.7629075
CTGACAT59600.07.75023841
GGGGAAT39750.07.74698831
Produced by FastQC (version 0.11.2)