FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22876783
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37600.09.40362746
TTAGTAA19850.09.3481349
TCGTATG37950.09.22441343
GGCGAAT39300.09.1708181
CTATTAG22650.09.11896946
GTTGGCT79700.09.0881491
GAAAAAT58650.09.0685751
TAATTCG30450.08.96998656
GTTAGTT23050.08.804821
TAACTAG31100.08.55529747
GCGATTT50750.08.4807161
CTATGCG31250.08.40374654
TATGCGG33900.08.05688355
GGAAAAT67300.08.0069871
AGTCACG45650.07.895641328
CCGTTAA35550.07.8723220
CTCGTAT45350.07.796442542
ATTAGTA23850.07.780258748
CGGGAAT39750.07.7465611
GTATGCC50000.07.70151345
Produced by FastQC (version 0.11.2)