FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18107568
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAT63000.015.3246546
GTCATAT67300.014.7090268
TAATTCG59300.014.34974156
TTAGTAA32850.014.0689849
AGTCATA70900.013.8634927
CTATTAG34900.013.64205946
GCCAGTA79800.013.5063091
ATTAGTA35800.013.30079448
AGTAGTC73450.013.2872694
TTAAGCC73800.013.08870829
GATTAAG73500.013.04448327
AACTAAT69650.012.56819653
TATTAGT37600.012.47695447
ACTAATT71000.012.42802954
TCATATG81400.012.2041549
GAACTAA70600.012.20059252
TAAGCCA81050.011.87499130
AGATTAA81850.011.75574326
ATTAAGC83600.011.67865428
TATGCTT84550.011.41878812
Produced by FastQC (version 0.11.2)