FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28570594
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG60050.013.41210156
TAGTCAT78000.011.5706886
GTCATAT81200.011.5454638
AGTCATA82550.011.356637
TTAAGCC79000.011.20778729
TTAGTAA41400.011.16331649
GATTAAG85300.010.58305727
CTATTAG44450.010.31718746
AGTAGTC93950.09.9041374
ATTAGTA46400.09.88475848
TTCGAAC86600.09.86739759
ACTAATT85750.09.63702954
GCCAGTA113700.09.6329171
ATTAAGC94250.09.50447228
GAACTAA84450.09.49490152
AACTAAT86900.09.46900253
TAAGCCA93050.09.44050430
AGATTAA98000.09.4254426
TATTAGT51050.09.12073547
TCATATG108500.08.8983999
Produced by FastQC (version 0.11.2)