FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18897728
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346160.18317545897580917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG28750.015.70379432
TAATTCG47500.015.70191456
TAGTCAT60850.015.2921726
GTCATAT65800.014.5136568
CACTTAG31650.014.15420631
GCCAGTA80900.013.7982381
AGTCATA68500.013.5841097
TTAAGCC68200.013.54733629
GATTAAG70600.013.18367727
TATGCCG34450.013.00719946
GAACTAA61700.012.59845152
AGTAGTC75300.012.5899714
TCATATG80300.012.3720859
AACTAAT65450.012.14400953
ACTAATT66750.012.11728954
TTAGGCA36550.011.970654534
TTCGAAC63900.011.94697459
TAAGCCA78550.011.89611430
AGATTAA78550.011.84886226
ATTAAGC79300.011.82651628
Produced by FastQC (version 0.11.2)