FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21942806
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAA40700.016.00112349
TAGTCAT70900.014.5055776
TAATTCG63600.014.37056356
CTATTAG45250.014.00298546
ATTAGTA46250.013.85349748
GCCAGTA89850.013.4363831
GTCATAT78900.013.3006928
TATTAGT48350.013.25073547
AGTCATA83600.012.343717
ATGCGTG57500.012.1673536
AGTAGTC83750.012.0294994
CTATTAA48700.011.9956412
TTAAGCC79850.011.92079129
ACTAATT86500.011.57757254
AGATTAA89050.011.47302326
GAACTAA82100.011.472652
AACTAAT82800.011.46039653
GATTAAG86100.011.46029227
GTAACAC56950.011.37445852
GTCCCTA51750.011.35609158
Produced by FastQC (version 0.11.2)