FastQCFastQC Report
Thu 15 Dec 2016
H5NFLBGXY_n01_b73_splitroot_aug2016_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5NFLBGXY_n01_b73_splitroot_aug2016_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23049041
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327510.14209267969109865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT43400.09.5945371
CTCGGTT65050.09.5750141
TCGCTTA40500.09.5010892
CTCGAAT61600.09.3728131
CTCGATT62600.09.2789851
CTGACAT105700.09.2031521
CTCGTAT43550.08.9186991
ACGGGTT49300.08.79859520
CTGGAAT122850.08.6874321
GGAAAAT117200.08.568821
CTCGCTT99300.08.492481
ATGCGTG53250.08.47416
CGCTTAT43850.08.45593553
CTGGGAT96650.08.3632841
CTCGTTT82000.08.2358831
GTCCGAT38850.08.1962861
CTCGGGT60350.08.1753921
CTCGGAT72950.08.1543681
GGAAGAT177300.08.1312111
GTCAAAT89850.07.9447241
Produced by FastQC (version 0.11.2)