FastQCFastQC Report
Sat 27 Apr 2024
H5N3TDSXC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5N3TDSXC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11371295829
Sequences flagged as poor quality0
Sequence length24
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGCCGTATCAT6540698265.751937473405081Illumina Single End PCR Primer 1 (100% over 24bp)
AGATCTCGGTGGTCGCCGTATAAT1091313380.9597088989777615Illumina Single End PCR Primer 1 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGG928144380.8162168973152303No Hit
CGCATCGGAGGCCATCGTGTAGAT235862800.20741945645146578No Hit
AACCGCTCTCTGGCACGTGTAGAT206342690.18145925768087764No Hit
GGTCTAAACTTCGCCTGTGTAGAT188893100.16611396171601614No Hit
GTAGGCGCTGAGTGACGTGTAGAT146004930.12839779405584226No Hit
CTGGTGGACTTCGTTTGTGTAGAT143057870.12580612812408085No Hit
AGATGTAGAGGAGTCAGTGTAGAT138741830.12201057125448213No Hit
AGTTTCACTCGTTATGGTGTAGAT136613220.12013865618692103No Hit
GGGGGGGGGGGGGGGGGGGGGTGG134746500.11849704908420249No Hit
TCCTGTGACAAGTTAGGTGTAGAT114193070.1004222137188407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)