FastQCFastQC Report
Fri 26 Apr 2024
H5N3TDSXC_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5N3TDSXC_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11371295829
Sequences flagged as poor quality0
Sequence length34
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGGATATCTCGTATGCCGTCTTCTGCTTGAA136626858512.015064998270745RNA PCR Primer, Index 31 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10571449809.296609602785843No Hit
TCGCTTCGATCTCGTATGCCGTCTTCTGCTTGAA8060381347.088357792472308TruSeq Adapter, Index 21 (96% over 28bp)
AACAAGATATCTCGTATGCCGTCTTCTGCTTGAA4257117563.743740048643492RNA PCR Primer, Index 38 (96% over 28bp)
CTCCTCTAATCTCGTATGCCGTCTTCTGCTTGAA3684221803.2399313634987834RNA PCR Primer, Index 13 (96% over 28bp)
GTAGGAACTGAGGCGGAGACGGTGCCGTTTAGAT3488862943.0681313655585485No Hit
GTAGGAACTGAGGCGGAGACGGTGAGTAAACCAT2855544922.5111869068761346No Hit
GTATGCTCATCTCGTATGCCGTCTTCTGCTTGAA2602300182.288481646360306Illumina PCR Primer Index 7 (96% over 28bp)
AAGCGATCGAGGCGGAGACGGTGAGTAAACCATC1993668581.7532466044156418No Hit
GCGTAATGTGAGGCGGAGACGGTGAGTAAACCAT1986388691.7468446163665452No Hit
AAGCGATCGAGGCGGAGACGGTGCCGTTTAGATC1016633960.8940352755640195No Hit
GCGTAATGTGAGGCGGAGACGGTGCCGTTTAGAT977028500.859205946879249No Hit
GTAGGAACTGAGGCGGAGACGGTGTTACGGTTAT928308320.816361067339883No Hit
GTGATACGGAGGCGGAGACGGTGAGTAAACCATC775860410.6822972699569894No Hit
GAACAGCTATGAGGCGGAGACGGTGAGTAAACCA536979970.47222407901001934No Hit
GTGATACGGAGGCGGAGACGGTGCCGTTTAGATC486593660.42791399266831964No Hit
CCAGTTGTATGAGGCGGAGACGGTGAGTAAACCA427028330.37553180958581495No Hit
CCATAGGATGAGGCGGAGACGGTGAGTAAACCAT403703680.3550199432596258No Hit
CGGTAAGTATGAGGCGGAGACGGTGAGTAAACCA336786900.2961728417451763No Hit
CCTTGAACGAGGCGGAGACGGTGAGTAAACCATC305307790.2684898841707902No Hit
ACGCAACTTGAGGCGGAGACGGTGAGTAAACCAT293791300.2583621993640774No Hit
CCAGTTGTATGAGGCGGAGACGGTGCCGTTTAGA243167240.21384303394856302No Hit
GAACAGCTATGAGGCGGAGACGGTGCCGTTTAGA215111200.1891703489512655No Hit
CGTGACATGAGGCGGAGACGGTGAGTAAACCATC209223760.18399289152817624No Hit
AGACGACAGAGGCGGAGACGGTGAGTAAACCATC204652820.17997317375041622No Hit
TGCGGTATGAGGCGGAGACGGTGAGTAAACCATC193319350.17000643805869628No Hit
GTGATACGGAGGCGGAGACGGTGTTACGGTTATC192666040.16943191250784934No Hit
CTTGCACTATGAGGCGGAGACGGTGAGTAAACCA161886100.14236381010081978No Hit
ACGCAACTTGAGGCGGAGACGGTGCCGTTTAGAT158303290.1392130610095308No Hit
CACTATCGTGAGGCGGAGACGGTGAGTAAACCAT156267730.13742297478663196No Hit
AAGCGATCGAGGCGGAGACGGTGTTACGGTTATC153935250.13537177496290428No Hit
GAACAGCTATGAGGCGGAGACGGTGTTACGGTTA149404410.13138732141589066No Hit
AGTAGGCTGAGGCGGAGACGGTGAGTAAACCATC147723560.12990916973882907No Hit
GAACGTGAATGAGGCGGAGACGGTGAGTAAACCA139088840.12231573436449025No Hit
GCGTAATGTGAGGCGGAGACGGTGTTACGGTTAT138751550.12201911909295735No Hit
AATGTGCCTGAGGCGGAGACGGTGAGTAAACCAT138259740.12158661781307176No Hit
TCTTCTCGCGTGCGGCGGAGAGGGTGAGTAAACC133763630.11763270607986923No Hit
GGATGGAATGAGGCGGAGACGGTGAGTAAACCAT132559650.11657391733837022No Hit
CCTTGAACGAGGCGGAGACGGTGCCGTTTAGATC129098060.11352976999399107No Hit
GATCGAAGGAGGCGGAGACGGTGAGTAAACCATC124922440.10985769949051248No Hit
CCATAGGATGAGGCGGAGACGGTGCCGTTTAGAT123472680.10858277003497699No Hit
CTTAGTCCATGAGGCGGAGACGGTGAGTAAACCA123419800.10853626697956871No Hit
GTCCTTGTTGAGGCGGAGACGGTGAGTAAACCAT120095070.10561247531149776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)