H5N3TDSXC_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5N3TDSXC_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11371295829
Sequences flagged as poor quality	0
Sequence length	125
%GC	60

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGTTGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGCT	266487817	2.343513184490207	RNA PCR Primer, Index 36 (96% over 26bp)
AACGCTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	243860523	2.144527120454356	Illumina PCR Primer Index 1 (95% over 24bp)
TGGACAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	209272412	1.840356764497293	Illumina PCR Primer Index 1 (95% over 24bp)
GTTCAAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGCTT	189258566	1.6643535516624013	RNA PCR Primer, Index 36 (96% over 27bp)
CTTCTGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	175002484	1.538984532912199	Illumina PCR Primer Index 1 (95% over 24bp)
GTTCAAGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	98953839	0.8702072348486433	RNA PCR Primer, Index 36 (95% over 24bp)
TCCTATGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	88676452	0.7798271484051107	RNA PCR Primer, Index 36 (95% over 24bp)
ACTTACCGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	80736507	0.7100026963866266	Illumina PCR Primer Index 1 (95% over 23bp)
AAGCTAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGCTT	64926531	0.5709686211347972	Illumina PCR Primer Index 1 (96% over 26bp)
ACACTGTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	57152285	0.5026013381363768	RNA PCR Primer, Index 36 (95% over 24bp)
TCACGTTCGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	40058742	0.3522794816210739	RNA PCR Primer, Index 36 (95% over 23bp)
AGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAA	37484410	0.32964061936023353	RNA PCR Primer, Index 36 (96% over 28bp)
ACAACTGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	34503420	0.30342557716251284	RNA PCR Primer, Index 36 (95% over 24bp)
GATGTAGCGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	34039264	0.29934375564472004	RNA PCR Primer, Index 36 (95% over 23bp)
CGAGAAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	33381185	0.2935565612044724	RNA PCR Primer, Index 36 (96% over 25bp)
GCTAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGCTT	32880485	0.28915336910104406	RNA PCR Primer, Index 36 (96% over 27bp)
ATGTCACGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	32615372	0.28682194615693346	RNA PCR Primer, Index 36 (95% over 24bp)
GTTCAAGGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	31497264	0.2769892233361226	RNA PCR Primer, Index 36 (95% over 23bp)
TCCTATGGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	29833450	0.2623575223847076	Illumina PCR Primer Index 1 (95% over 22bp)
TGTTGGAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	27534117	0.24213702126876574	RNA PCR Primer, Index 36 (95% over 24bp)
AGGCAGTTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	25912691	0.22787808346270752	RNA PCR Primer, Index 36 (95% over 23bp)
AAGCTAGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	25612206	0.2252355965859377	RNA PCR Primer, Index 36 (95% over 24bp)
TAGTGACCGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	23042536	0.20263773229111723	Illumina PCR Primer Index 1 (95% over 22bp)
TTCCATCCGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	22315312	0.1962424717074872	RNA PCR Primer, Index 36 (95% over 23bp)
AGCCTACTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	22175745	0.19501510939013317	Illumina PCR Primer Index 1 (95% over 22bp)
AGTGCAAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGCT	22033472	0.19376395031257965	RNA PCR Primer, Index 36 (96% over 26bp)
ACAAGGACGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	21485243	0.1889427847370446	RNA PCR Primer, Index 36 (95% over 23bp)
ACACTGTGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	20761988	0.1825824278271883	RNA PCR Primer, Index 36 (95% over 23bp)
GGACTAAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	20040481	0.1762374429560714	RNA PCR Primer, Index 36 (95% over 24bp)
GCTGTGTTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	19464312	0.1711705709947369	Illumina PCR Primer Index 1 (95% over 22bp)
CTCTCCTTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	19433346	0.17089825374553624	Illumina PCR Primer Index 1 (95% over 22bp)
AGTTGCGTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	19213803	0.1689675766854944	Illumina PCR Primer Index 1 (95% over 22bp)
AGTTGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTGGATA	18674482	0.164224748707837	No Hit
GGACTAAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGCT	18381849	0.16165131288837917	RNA PCR Primer, Index 36 (96% over 26bp)
AGTGCAAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	17627947	0.15502144403845144	RNA PCR Primer, Index 36 (95% over 24bp)
TGGACAGAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	17165610	0.15095561893854587	RNA PCR Primer, Index 36 (95% over 23bp)
AACGCTGAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	17119762	0.15055242830232063	RNA PCR Primer, Index 36 (95% over 23bp)
TGCAGAACGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	17044949	0.14989451735597795	RNA PCR Primer, Index 36 (95% over 23bp)
GAGAGCAAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	15891459	0.13975064266178278	Illumina PCR Primer Index 1 (95% over 24bp)
CTTCTGGAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	15878814	0.13963944161495262	RNA PCR Primer, Index 36 (95% over 23bp)
ACTTACCGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	15493848	0.13625402269885842	RNA PCR Primer, Index 36 (95% over 23bp)
GCTAGAGTGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	15320514	0.1347297109352162	RNA PCR Primer, Index 36 (95% over 23bp)
AGTGATCCGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	14838568	0.13049144286755324	RNA PCR Primer, Index 36 (95% over 23bp)
CTGGCATAGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	14766367	0.12985650203859453	Illumina PCR Primer Index 1 (95% over 22bp)
TGCGACAAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	13709261	0.12056023522875495	Illumina PCR Primer Index 1 (95% over 24bp)
ACAACTGGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	13654390	0.12007769567631393	RNA PCR Primer, Index 36 (95% over 23bp)
CGAATGCAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	12899246	0.11343690458833458	Illumina PCR Primer Index 1 (95% over 24bp)
TCACGTTCAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTG	12284961	0.1080348377593862	Illumina PCR Primer Index 1 (95% over 23bp)
GCGTCTAAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCTGC	12131135	0.10668208076217837	RNA PCR Primer, Index 36 (96% over 25bp)
CTTCGATCGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	11591925	0.10194022892656907	Illumina PCR Primer Index 1 (95% over 22bp)
AAGCTAGGGAGGCGGAGACGGTGAGTAAACCATCTCGTATGCCGTCTTCT	11499686	0.10112907247274817	Illumina PCR Primer Index 1 (95% over 22bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content