FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah9041i-031517.35100000080fec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah9041i-031517.35100000080fec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21885789
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG6084192.7799728856017025TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA692600.043.6360171
GCACACG696700.042.81443811
ACACGTC698800.042.53757513
CACACGT704950.042.33881812
CACGTCT700850.042.3361114
ACGTCTG700450.042.29993415
AGTCACG697950.042.23604228
AGCACAC710100.042.1332410
TCGGAAG716200.042.1126373
GAGCACA711300.042.1001179
CGTCTGA707250.041.9222416
GTCACGT703400.041.8491729
AGAGCAC718400.041.7278828
CGATCTC693750.041.65100540
ATCGGAA728850.041.4990542
CAGTCAC709550.041.4913627
TCCAGTC709850.041.4323425
GATCTCG700800.041.43076741
CACGTGA710600.041.36498331
TCACGTG712500.041.30520230
Produced by FastQC (version 0.11.2)