FastQCFastQC Report
Thu 16 Mar 2017
H5M3MBCXY_l02n01_ah9041a-031517.35100000080fc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5M3MBCXY_l02n01_ah9041a-031517.35100000080fc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404819
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG12800.3161906926305337TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG1600.037.96750328
GATCGGA1700.037.0667571
CACACGT1700.037.05760212
TCACGTC1800.036.24880630
CACGTCT1750.035.99881714
CCGCACA1750.035.99881736
TCCGCAC1800.034.99884835
GCACACG1800.034.99884811
GTCACGT1800.034.99884829
ACGTCCG1800.034.99884832
CATCTCG1850.034.05293341
CGTCTGA1850.034.05293316
ACGTCTG1850.034.05293315
CACGTCC1900.033.15680331
CGTCCGC1900.033.15680333
GAGCACA2000.032.6239249
GAACTCC2050.031.8282221
GTCCGCA2050.031.8282234
AGCACAC2000.031.49896210
ATCTCGT2000.031.49896242
Produced by FastQC (version 0.11.2)